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The AQC derivatives ended up stable for days. Optimum fluorescence was obtained with an excitation wavelength regarding 246 nm and an release wavelength involving 390 nm. Chromatography ended up being carried out employing a C18 ray as well as slope elution with A single milliliters min1 along with methanol and 3.05 mol L1 phosphate load at pH Four. The particular restriction involving detection ended up being Six mu g L1 for both FB1 and FB2. The strategy ended up being efficiently put on the determination of FB1 along with FB2 within